Upload 16s amplicon sequencing raw data to NCBI

Introduction to 16S and ITS rRNA Sequencing

16S and Internal Transcribed Spacer (ITS) ribosomal RNA (rRNA) sequencing are common amplicon sequencing methods used to identify and compare bacteria or fungi present within a given sample. Both ITS and 16S rRNA gene sequencing are well-established methods for comparing sample phylogeny and taxonomy from complex microbiomes or environments that are difficult or impossible to study.

register a NCBI account

click submit

add bioproject

choose project type and next steps in adding bioproject

choose raw data sequences and metagenome

sample name should contain “metagenomes”

don’t add biosample

add biosample

choose batch biosamples

choose single Amplified genome sequences

fill the downloaded metadata file in Excel format and submit

upload sequence read archive

fill the bioprogect accession

fill the downloaded metadata file in Excel format and submit. Sequences
of different sample should be splited to different files. Every fastq
file or gz file should have a corresponding biosample.

you should install aspera plugin before upload to speed up the data
transmission