Key Words: Wheat, Nitrogen deficiency stress, Rhizosphere microorganism, Soil microorganism, Metagenomics, Metaproteomics
- Wheat is an important grain crop in China, and nitrogen is an essential nutrient element for the growth and development of wheat. However, the utilization efficiency of nitrogen fertilizer applied in the field is very low. The loss of nitrogen fertilizer is driven by soil microorganisms, and the input of nitrogen fertilizer is also one of the main driving forces for the change of soil microbial community structure. In addition, plants directly obtain mineral nutrients from the rhizosphere environment, and enzymes secreted by rhizosphere microorganisms can catalyze the decomposition of complex organic matters in the soil into simple inorganic substances for utilization by plants. Rhizosphere microorganisms are essential to the healthy growth of plants. We set up an root chamber experiment of wheat with different distances from root to bulk soil and different nitrogen input. We combined the traditional plant and soil physio-biochemistry experimental methods and soil multi-omics methods of genomics, proteomics to study how the wheat rhizosphere microorganisms change under nitrogen deficiency stress.
16S and Internal Transcribed Spacer (ITS) ribosomal RNA (rRNA) sequencing are common amplicon sequencing methods used to identify and compare bacteria or fungi present within a given sample. Both ITS and 16S rRNA gene sequencing are well-established methods for comparing sample phylogeny and taxonomy from complex microbiomes or environments that are difficult or impossible to study.